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untouched splenic cd8 t splenocytes  (Miltenyi Biotec)


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    Miltenyi Biotec untouched splenic cd8 t splenocytes
    Untouched Splenic Cd8 T Splenocytes, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 629 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+cd8a+t+cell+isolation+kit/CD8a%2B+T+Cell+Isolation+Kit%2C+mouse/10__1158_slash_1535___7163__mct___25___1488-143-7-26
    Average 97 stars, based on 629 article reviews
    untouched splenic cd8 t splenocytes - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: MFAP4 activates PI3K/AKT-NF-κB signaling to drive tumor progression and immune evasion in bladder cancer.
    Article Snippet: .. CD8a+ T cells were then isolated by magnetic negative selection using the Mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130–104-075) according to the manufacturer's instructions. ..

    Article Title: B cells maintain the homeostasis of splenic marginal zone antigen-presenting cells to promote the antiviral CD8 + T-cell response
    Article Snippet: .. OVA-specific T-cell transgenic (TCR) CD8 + T-cell isolation was performed on the spleen and peripheral LNs of OT-I mice via the Mouse CD8a + T-Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions. .. For the cDC1/OVA or SIINFEKL/OT-I coculture experiments, purified 4 × 10 4 XCR1 + cDC1s, CD103 high cDC1s or CD103 - cDC1s were incubated with OVA or the SIINFEKL peptide for 60 min at 37 °C in a cell culture incubator; subsequently, XCR1 + cDC1s, CD103 high cDC1s or CD103 - cDC1s were extensively washed and cultured with 1 × 10 5 Trace Violet (Thermo Fisher)-labeled OT-I CD8 + T cells.

    Article Title: A hybrid polymeric system for pulmonary mRNA delivery: Advancing mucosal vaccine development
    Article Snippet: .. The isolation of CD8 + T cells was performed using a mouse CD8a + T cell isolation kit purchased from Miltenyi. ..

    Article Title: Type I interferon drives T cell responses to amyloid beta in the central nervous system
    Article Snippet: .. CD8+ T cells were isolated from spleens of Rosa26-Cas9 −/− mice (GFP-) using mouse CD8a+ T-cell isolation kit (Miltenyi Biotec) for isolating untouched CD8 T-cells according to the manufacturer’s instructions. ..

    Article Title: Process for the preparation of lipidated proteinaceous structures
    Article Snippet: .. Spleens were mashed and CD8+ cells isolated after red cell lysis (ACK lysing buffer, #A1049201, Thermo Fisher Scientific) using a mouse CD8a+ T cell isolation kit (#130-104-075, Miltenyi Biotec, Bergisch-Gladbach, Germany) and magnetic cell isolation (LS columns, #130-042-401, Miltenyi Biotec) used according to manufacturer's instructions. .. Purified CD8+ T cells were stained by 1 nM Cell Tracer Far Red (#C34564, Thermo Fisher Scientific) for 5 min at 35° C. and washed with FACS buffer.

    Article Title: Loss of the autoimmune risk gene TREX1 reveals a convergence of mechanisms promoting immune tolerance loss and antitumor immunity
    Article Snippet: .. Murine splenic CD8+ T cells were isolated using a mouse CD8a T cell isolation kit (Miltenyi Biotec, 130-104-075) and were cultured in complete T cell medium containing RPMI 1640 (Gibco, 31800022), 10% FBS (Gibco, 16140071), 1% GlutaMAX (Gibco), 1% Hepes, 1 mM sodium pyruvate (Gibco, 11360070), 1% nonessential amino acids (Gibco, 11140050), penicillin/streptomycin (Gibco, 15140122), and 55 μM 2-mercaptoethanol (Gibco, 21985023). .. CD8+ T cells were supplemented with mouse interleukin-7 (5 ng/ml; Miltenyi Biotec, 130-094-066) and incubated overnight at 37°C.

    Selection:

    Article Title: MFAP4 activates PI3K/AKT-NF-κB signaling to drive tumor progression and immune evasion in bladder cancer.
    Article Snippet: .. CD8a+ T cells were then isolated by magnetic negative selection using the Mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130–104-075) according to the manufacturer's instructions. ..

    Cell Isolation:

    Article Title: MFAP4 activates PI3K/AKT-NF-κB signaling to drive tumor progression and immune evasion in bladder cancer.
    Article Snippet: .. CD8a+ T cells were then isolated by magnetic negative selection using the Mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130–104-075) according to the manufacturer's instructions. ..

    Article Title: A hybrid polymeric system for pulmonary mRNA delivery: Advancing mucosal vaccine development
    Article Snippet: .. The isolation of CD8 + T cells was performed using a mouse CD8a + T cell isolation kit purchased from Miltenyi. ..

    Article Title: Functional nutrient-genetic profiling reveals biotin and FBXW7 are essential to bypass glutamine addiction.
    Article Snippet: Spleens were extracted from three 10 weeks-old males, and splenocytes were activated on plate-bound 0.5 μg/ml hamster anti-mouse CD3 clone eBio500A2 (ThermoFisher, 16-0033-86) and 1μg/ml hamster anti-mouse CD28 clone 37.51 (ThermoFisher, 16-0281-82). .. CD8 + T cells were purified 48 h post-stimulation using the mouse CD8a + T cell Isolation Kit (MACS Miltenyi Biotec, 130-104-075), following manufacturer’s instructions. .. Primary CD8 + T cells were cultured in RPMI medium with L-glutamine and sodium bicarbonate (Sigma-Aldrich, 1640) supplemented with 10% heat-inactivated FBS, 100 U/mL penicillin/streptomycin, 1 mM sodium pyruvate, 50 μM β-mercaptoethanol (PAN Biotech, P07-05020) and 100 U/mL murine IL-2 (ThermoFisher/Gibco, 212-12).

    Article Title: Process for the preparation of lipidated proteinaceous structures
    Article Snippet: .. Spleens were mashed and CD8+ cells isolated after red cell lysis (ACK lysing buffer, #A1049201, Thermo Fisher Scientific) using a mouse CD8a+ T cell isolation kit (#130-104-075, Miltenyi Biotec, Bergisch-Gladbach, Germany) and magnetic cell isolation (LS columns, #130-042-401, Miltenyi Biotec) used according to manufacturer's instructions. .. Purified CD8+ T cells were stained by 1 nM Cell Tracer Far Red (#C34564, Thermo Fisher Scientific) for 5 min at 35° C. and washed with FACS buffer.

    Article Title: Scalable generation of pure CD103+ cDC1 from iDC1 cultures
    Article Snippet: .. CD8+ T cells were purified from spleens and lymph nodes of CD45.1+ OT-I mice using the Mouse CD8a+ T cell Isolation Kit (Miltenyi Biotec #130-104-075) and LS columns according to the manufacturer’s instructions. .. Purified CD8+ T cells were washed with sterile PBS and labeled with 5 μM CellTrace Violet (CTV; Invitrogen #C34557) for 20 min at 37 °C according to the manufacturer’s instructions.

    Article Title: Loss of the autoimmune risk gene TREX1 reveals a convergence of mechanisms promoting immune tolerance loss and antitumor immunity
    Article Snippet: .. Murine splenic CD8+ T cells were isolated using a mouse CD8a T cell isolation kit (Miltenyi Biotec, 130-104-075) and were cultured in complete T cell medium containing RPMI 1640 (Gibco, 31800022), 10% FBS (Gibco, 16140071), 1% GlutaMAX (Gibco), 1% Hepes, 1 mM sodium pyruvate (Gibco, 11360070), 1% nonessential amino acids (Gibco, 11140050), penicillin/streptomycin (Gibco, 15140122), and 55 μM 2-mercaptoethanol (Gibco, 21985023). .. CD8+ T cells were supplemented with mouse interleukin-7 (5 ng/ml; Miltenyi Biotec, 130-094-066) and incubated overnight at 37°C.

    Transgenic Assay:

    Article Title: B cells maintain the homeostasis of splenic marginal zone antigen-presenting cells to promote the antiviral CD8 + T-cell response
    Article Snippet: .. OVA-specific T-cell transgenic (TCR) CD8 + T-cell isolation was performed on the spleen and peripheral LNs of OT-I mice via the Mouse CD8a + T-Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions. .. For the cDC1/OVA or SIINFEKL/OT-I coculture experiments, purified 4 × 10 4 XCR1 + cDC1s, CD103 high cDC1s or CD103 - cDC1s were incubated with OVA or the SIINFEKL peptide for 60 min at 37 °C in a cell culture incubator; subsequently, XCR1 + cDC1s, CD103 high cDC1s or CD103 - cDC1s were extensively washed and cultured with 1 × 10 5 Trace Violet (Thermo Fisher)-labeled OT-I CD8 + T cells.

    Purification:

    Article Title: Functional nutrient-genetic profiling reveals biotin and FBXW7 are essential to bypass glutamine addiction.
    Article Snippet: Spleens were extracted from three 10 weeks-old males, and splenocytes were activated on plate-bound 0.5 μg/ml hamster anti-mouse CD3 clone eBio500A2 (ThermoFisher, 16-0033-86) and 1μg/ml hamster anti-mouse CD28 clone 37.51 (ThermoFisher, 16-0281-82). .. CD8 + T cells were purified 48 h post-stimulation using the mouse CD8a + T cell Isolation Kit (MACS Miltenyi Biotec, 130-104-075), following manufacturer’s instructions. .. Primary CD8 + T cells were cultured in RPMI medium with L-glutamine and sodium bicarbonate (Sigma-Aldrich, 1640) supplemented with 10% heat-inactivated FBS, 100 U/mL penicillin/streptomycin, 1 mM sodium pyruvate, 50 μM β-mercaptoethanol (PAN Biotech, P07-05020) and 100 U/mL murine IL-2 (ThermoFisher/Gibco, 212-12).

    Article Title: Scalable generation of pure CD103+ cDC1 from iDC1 cultures
    Article Snippet: .. CD8+ T cells were purified from spleens and lymph nodes of CD45.1+ OT-I mice using the Mouse CD8a+ T cell Isolation Kit (Miltenyi Biotec #130-104-075) and LS columns according to the manufacturer’s instructions. .. Purified CD8+ T cells were washed with sterile PBS and labeled with 5 μM CellTrace Violet (CTV; Invitrogen #C34557) for 20 min at 37 °C according to the manufacturer’s instructions.

    Magnetic Cell Separation:

    Article Title: Functional nutrient-genetic profiling reveals biotin and FBXW7 are essential to bypass glutamine addiction.
    Article Snippet: Spleens were extracted from three 10 weeks-old males, and splenocytes were activated on plate-bound 0.5 μg/ml hamster anti-mouse CD3 clone eBio500A2 (ThermoFisher, 16-0033-86) and 1μg/ml hamster anti-mouse CD28 clone 37.51 (ThermoFisher, 16-0281-82). .. CD8 + T cells were purified 48 h post-stimulation using the mouse CD8a + T cell Isolation Kit (MACS Miltenyi Biotec, 130-104-075), following manufacturer’s instructions. .. Primary CD8 + T cells were cultured in RPMI medium with L-glutamine and sodium bicarbonate (Sigma-Aldrich, 1640) supplemented with 10% heat-inactivated FBS, 100 U/mL penicillin/streptomycin, 1 mM sodium pyruvate, 50 μM β-mercaptoethanol (PAN Biotech, P07-05020) and 100 U/mL murine IL-2 (ThermoFisher/Gibco, 212-12).

    Lysis:

    Article Title: Process for the preparation of lipidated proteinaceous structures
    Article Snippet: .. Spleens were mashed and CD8+ cells isolated after red cell lysis (ACK lysing buffer, #A1049201, Thermo Fisher Scientific) using a mouse CD8a+ T cell isolation kit (#130-104-075, Miltenyi Biotec, Bergisch-Gladbach, Germany) and magnetic cell isolation (LS columns, #130-042-401, Miltenyi Biotec) used according to manufacturer's instructions. .. Purified CD8+ T cells were stained by 1 nM Cell Tracer Far Red (#C34564, Thermo Fisher Scientific) for 5 min at 35° C. and washed with FACS buffer.

    Cell Culture:

    Article Title: Loss of the autoimmune risk gene TREX1 reveals a convergence of mechanisms promoting immune tolerance loss and antitumor immunity
    Article Snippet: .. Murine splenic CD8+ T cells were isolated using a mouse CD8a T cell isolation kit (Miltenyi Biotec, 130-104-075) and were cultured in complete T cell medium containing RPMI 1640 (Gibco, 31800022), 10% FBS (Gibco, 16140071), 1% GlutaMAX (Gibco), 1% Hepes, 1 mM sodium pyruvate (Gibco, 11360070), 1% nonessential amino acids (Gibco, 11140050), penicillin/streptomycin (Gibco, 15140122), and 55 μM 2-mercaptoethanol (Gibco, 21985023). .. CD8+ T cells were supplemented with mouse interleukin-7 (5 ng/ml; Miltenyi Biotec, 130-094-066) and incubated overnight at 37°C.



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    Image Search Results


    GPCR68 as a pH-Sensing regulator in T Cells and generation of GPCR68 fl/fl CD4 Cre mice. (A) Schematic diagram of the effect of pH on T cell GPCR68 as well as tumor. (B) Naïve CD4 + T cells were isolated and activated using anti-CD3 and anti-CD28 using the culture media with varying pH. RT-qPCR was performed to determine the expression of GPCR68 at various pH. (C) Naïve CD4 + T cells were activated with anti-CD3 and anti-CD28 under different pH conditions, and GPCR68 protein expression was assessed by Western blot analysis. (D) To generate conditional knockout (CKO) of GPCR68 in T cells, GPCR68 fl/fl mice were crossed with CD4 Cre mice and generated GPCR68 fl/fl CD4 Cre (CKO). (E) Flow cytometry was used to determine the population of CD4 and CD8 cells in the lymph nodes (LN), thymus (THY), and spleen (SP) at the basal level in CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (F) Flow cytometry was used to determine the population of Foxp3+ Treg cells in the lymph nodes, thymus, and spleen at the basal level in the CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (G-H) The population of F4/80+, CD11c+ (G), and B220+ (H) cells was determined in the lymph nodes and spleen at the basal level in the CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (I-J) Flow cytometry was used to evaluate the CD4 + or CD8 + T cells for the determination of intracellular cytokines IFN-γ+ (I), or TNF-α+ (J) from the spleen and lymph nodes at basal level in the CD4 Cre or GPCR68 fl/fl CD4 Cre mice. Student t-test was performed for comparison between the two groups. Data are mean ± SEM (n = 5), ∗ p < 0.05.

    Journal: Bioactive Materials

    Article Title: pH-neutralization strategy to suppress GPCR68 spatiotemporally activates T cells and enhances anti-tumor immunity

    doi: 10.1016/j.bioactmat.2026.02.039

    Figure Lengend Snippet: GPCR68 as a pH-Sensing regulator in T Cells and generation of GPCR68 fl/fl CD4 Cre mice. (A) Schematic diagram of the effect of pH on T cell GPCR68 as well as tumor. (B) Naïve CD4 + T cells were isolated and activated using anti-CD3 and anti-CD28 using the culture media with varying pH. RT-qPCR was performed to determine the expression of GPCR68 at various pH. (C) Naïve CD4 + T cells were activated with anti-CD3 and anti-CD28 under different pH conditions, and GPCR68 protein expression was assessed by Western blot analysis. (D) To generate conditional knockout (CKO) of GPCR68 in T cells, GPCR68 fl/fl mice were crossed with CD4 Cre mice and generated GPCR68 fl/fl CD4 Cre (CKO). (E) Flow cytometry was used to determine the population of CD4 and CD8 cells in the lymph nodes (LN), thymus (THY), and spleen (SP) at the basal level in CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (F) Flow cytometry was used to determine the population of Foxp3+ Treg cells in the lymph nodes, thymus, and spleen at the basal level in the CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (G-H) The population of F4/80+, CD11c+ (G), and B220+ (H) cells was determined in the lymph nodes and spleen at the basal level in the CD4 Cre or GPCR68 fl/fl CD4 Cre mice. (I-J) Flow cytometry was used to evaluate the CD4 + or CD8 + T cells for the determination of intracellular cytokines IFN-γ+ (I), or TNF-α+ (J) from the spleen and lymph nodes at basal level in the CD4 Cre or GPCR68 fl/fl CD4 Cre mice. Student t-test was performed for comparison between the two groups. Data are mean ± SEM (n = 5), ∗ p < 0.05.

    Article Snippet: Naïve T cells were purified from lymph nodes as well as spleens of C57/BL6, CD4 Cre , GPCR68 fl/fl CD4 Cre (CKO) mice by using the mouse naïve CD4 + T Cell Isolation Kit (#130-104-453; Miltenyi Biotec) or naïve CD8 + T Cell Isolation Kit (#130-096-543; Miltenyi Biotec) for negative selection.

    Techniques: Isolation, Quantitative RT-PCR, Expressing, Western Blot, Knock-Out, Generated, Flow Cytometry, Comparison

    GPCR68 fl/fl CD4 Cre mice exhibit improved anti-tumor mmune responses. (A-C) Naïve CD4 + T cells were isolated from CD4 Cre or GPCR68 fl/fl CD4 Cre mice and activated using anti-CD3 and anti-CD28 using the culture media under physiologic neutral pH (7.4) or varying pH 6.0, 6.5, or 7.8. Flow cytometry plots showing the expression of IFN-γ and IL-2 in CD4 + T cells from CD4 Cre and GPCR68 fl/fl CD4 Cre mice. Each panel represents the frequency of IFN-γ + and IL-2 + cells. (B) Bar graph summarizing the percentage of IFN-γ + CD4 + T cells at each pH level for CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (C) Bar graph showing the percentage of IL-2 + CD4 + T cells at each pH for CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (D) Experimental timeline depicting tumor induction and treatment protocol in CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (E) Tumor growth curves in CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (F) Tumor weight in CD4 Cre versus GPCR68 fl/fl CD4 Cre mice at the time of harvesting on day 21. (G) Representative images of excised tumors at day 21. (H) Flow cytometric analysis of IFN-γ production by tumor-infiltrating CD4 + and CD8 + T cells. (I) Flow cytometric analysis of TNF-α production by tumor-infiltrating CD4 + and CD8 + T cells. Student t-test was performed for comparison between the two groups. Two-way ANOVA was used for multiple comparisons. Data are mean ± SEM (n = 5). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns = not significant.

    Journal: Bioactive Materials

    Article Title: pH-neutralization strategy to suppress GPCR68 spatiotemporally activates T cells and enhances anti-tumor immunity

    doi: 10.1016/j.bioactmat.2026.02.039

    Figure Lengend Snippet: GPCR68 fl/fl CD4 Cre mice exhibit improved anti-tumor mmune responses. (A-C) Naïve CD4 + T cells were isolated from CD4 Cre or GPCR68 fl/fl CD4 Cre mice and activated using anti-CD3 and anti-CD28 using the culture media under physiologic neutral pH (7.4) or varying pH 6.0, 6.5, or 7.8. Flow cytometry plots showing the expression of IFN-γ and IL-2 in CD4 + T cells from CD4 Cre and GPCR68 fl/fl CD4 Cre mice. Each panel represents the frequency of IFN-γ + and IL-2 + cells. (B) Bar graph summarizing the percentage of IFN-γ + CD4 + T cells at each pH level for CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (C) Bar graph showing the percentage of IL-2 + CD4 + T cells at each pH for CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (D) Experimental timeline depicting tumor induction and treatment protocol in CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (E) Tumor growth curves in CD4 Cre and GPCR68 fl/fl CD4 Cre mice. (F) Tumor weight in CD4 Cre versus GPCR68 fl/fl CD4 Cre mice at the time of harvesting on day 21. (G) Representative images of excised tumors at day 21. (H) Flow cytometric analysis of IFN-γ production by tumor-infiltrating CD4 + and CD8 + T cells. (I) Flow cytometric analysis of TNF-α production by tumor-infiltrating CD4 + and CD8 + T cells. Student t-test was performed for comparison between the two groups. Two-way ANOVA was used for multiple comparisons. Data are mean ± SEM (n = 5). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ns = not significant.

    Article Snippet: Naïve T cells were purified from lymph nodes as well as spleens of C57/BL6, CD4 Cre , GPCR68 fl/fl CD4 Cre (CKO) mice by using the mouse naïve CD4 + T Cell Isolation Kit (#130-104-453; Miltenyi Biotec) or naïve CD8 + T Cell Isolation Kit (#130-096-543; Miltenyi Biotec) for negative selection.

    Techniques: Isolation, Flow Cytometry, Expressing, Comparison

    Anti-tumor effects of borate bioactive glass (BOLT) in B16 tumor. (A) Schematic illustration depicting the induction of B16 melanoma tumors, followed by treatment with BOLT at various time points, and tumor harvesting for subsequent analysis. (B) Tumor growth curves showing tumor volume in Control and BOLT-treated B16 melanoma tumors in mice. (C) Tumor weight at the time of harvesting in the BOLT-treated group compared to the Control. (D) Representative images of excised tumors from Control and BOLT-treated mice. (E) In vivo imaging of tumor-bearing mice in both the Control and BOLT-treated groups. (F) Flow cytometry analysis showing IFN-γ production in CD4 + and CD8 + T cells following BOLT treatment compared to Control. (G) Flow cytometry analysis demonstrated TNF-α production in CD4 + and CD8 + T cells in the BOLT-treated group, with a significant increase observed in CD8 + T cells. Student t-test was performed for comparison between the two groups. Two-way ANOVA was used for multiple comparisons. Data represent the mean ± SEM (n = 5). ∗ p < 0.05, ∗∗ p < 0.01.

    Journal: Bioactive Materials

    Article Title: pH-neutralization strategy to suppress GPCR68 spatiotemporally activates T cells and enhances anti-tumor immunity

    doi: 10.1016/j.bioactmat.2026.02.039

    Figure Lengend Snippet: Anti-tumor effects of borate bioactive glass (BOLT) in B16 tumor. (A) Schematic illustration depicting the induction of B16 melanoma tumors, followed by treatment with BOLT at various time points, and tumor harvesting for subsequent analysis. (B) Tumor growth curves showing tumor volume in Control and BOLT-treated B16 melanoma tumors in mice. (C) Tumor weight at the time of harvesting in the BOLT-treated group compared to the Control. (D) Representative images of excised tumors from Control and BOLT-treated mice. (E) In vivo imaging of tumor-bearing mice in both the Control and BOLT-treated groups. (F) Flow cytometry analysis showing IFN-γ production in CD4 + and CD8 + T cells following BOLT treatment compared to Control. (G) Flow cytometry analysis demonstrated TNF-α production in CD4 + and CD8 + T cells in the BOLT-treated group, with a significant increase observed in CD8 + T cells. Student t-test was performed for comparison between the two groups. Two-way ANOVA was used for multiple comparisons. Data represent the mean ± SEM (n = 5). ∗ p < 0.05, ∗∗ p < 0.01.

    Article Snippet: Naïve T cells were purified from lymph nodes as well as spleens of C57/BL6, CD4 Cre , GPCR68 fl/fl CD4 Cre (CKO) mice by using the mouse naïve CD4 + T Cell Isolation Kit (#130-104-453; Miltenyi Biotec) or naïve CD8 + T Cell Isolation Kit (#130-096-543; Miltenyi Biotec) for negative selection.

    Techniques: Control, In Vivo Imaging, Flow Cytometry, Comparison

    Combinational treatment of BOLT and anti-CTLA-4 blockade enhances anti-tumor immune response in B16 melanoma. (A) C57BL/6 mice were subcutaneously injected with 1 × 10 5 B16 melanoma cells on day 0 to induce tumors. On day 7, mice were randomly divided into groups and treated with either BOLT alone (intratumoral injection administered on alternate days starting from day 7), anti-CTLA-4 (intraperitoneal injection administered on days 9, 11, 13, and 15), or a combination of both treatments. PBS was used as a vehicle Control, while IgG was used as anti-CTLA-4 Control. Tumor growth was monitored throughout the treatment period, and tumors were harvested for analysis on day 21. (B-C) Tumor growth curves and area under the curve (AUC) analysis for WT mice treated with BOLT, with or without anti-CTLA-4 antibody, following subcutaneous injection of B16 melanoma cells. Tumor growth was monitored, and analysis was conducted on day 21. (D) Representative images of excised tumors at day 21, showed reduced tumor size in combination-treated mice. (E, F) Flow cytometry analysis of IFN-γ production by tumor-infiltrating CD4 + and CD8 + T cells. (G, H) Flow cytometry analysis of TNF-α production by tumor-infiltrating CD4 + and CD8 + T cells. Two-way ANOVA was used for multiple comparisons. Data are mean ± SEM (n = 5), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Journal: Bioactive Materials

    Article Title: pH-neutralization strategy to suppress GPCR68 spatiotemporally activates T cells and enhances anti-tumor immunity

    doi: 10.1016/j.bioactmat.2026.02.039

    Figure Lengend Snippet: Combinational treatment of BOLT and anti-CTLA-4 blockade enhances anti-tumor immune response in B16 melanoma. (A) C57BL/6 mice were subcutaneously injected with 1 × 10 5 B16 melanoma cells on day 0 to induce tumors. On day 7, mice were randomly divided into groups and treated with either BOLT alone (intratumoral injection administered on alternate days starting from day 7), anti-CTLA-4 (intraperitoneal injection administered on days 9, 11, 13, and 15), or a combination of both treatments. PBS was used as a vehicle Control, while IgG was used as anti-CTLA-4 Control. Tumor growth was monitored throughout the treatment period, and tumors were harvested for analysis on day 21. (B-C) Tumor growth curves and area under the curve (AUC) analysis for WT mice treated with BOLT, with or without anti-CTLA-4 antibody, following subcutaneous injection of B16 melanoma cells. Tumor growth was monitored, and analysis was conducted on day 21. (D) Representative images of excised tumors at day 21, showed reduced tumor size in combination-treated mice. (E, F) Flow cytometry analysis of IFN-γ production by tumor-infiltrating CD4 + and CD8 + T cells. (G, H) Flow cytometry analysis of TNF-α production by tumor-infiltrating CD4 + and CD8 + T cells. Two-way ANOVA was used for multiple comparisons. Data are mean ± SEM (n = 5), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

    Article Snippet: Naïve T cells were purified from lymph nodes as well as spleens of C57/BL6, CD4 Cre , GPCR68 fl/fl CD4 Cre (CKO) mice by using the mouse naïve CD4 + T Cell Isolation Kit (#130-104-453; Miltenyi Biotec) or naïve CD8 + T Cell Isolation Kit (#130-096-543; Miltenyi Biotec) for negative selection.

    Techniques: Injection, Control, Flow Cytometry